LightBox : A multiwell plate illumination system for photoactive molecule characterization

Multiwell plates (MWPs) are the workhorses of life sciences. However, biophotonics research with MWPs is limited, in part due to lack equipment suitable for photo-irradiation photoactive molecules a MWP-suitable, high-throughput manner, either commercially or through open-source MWP systems. Here we present “LightBox”, calibrated controllable illumination system broad applications including screening and characterization photocatalytic chemicals. LightBox high intensity, accurately controllable, uniform designed electronics control unit that provides simple intuitive interface. can reach intensities 0.23 mW/mm2 at wavelengths 405 nm variance between well sites <5%. The usefulness demonstrated by assessing IC50 photosensitizing compound using live/dead assay following simultaneous irradiation sample range concentrations, eliminating uncontrolled variables concentrations drastically increasing assessment speed.